Journal: iScience

Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth

doi: 10.1016/j.isci.2023.108341

Figure Lengend Snippet: Local immune responses in the cervix with signs of early premature labor or active premature labor (A) Dams were intra-amniotically injected under ultrasound guidance with PBS, LPS, or IL-1α, or subcutaneously with DMSO or RU-486 (n = 3 dams per group). The cervix was collected with signs of early premature labor (SEL; 6 h) and histology was utilized to evaluate structure by Movat pentachrome staining, leukocyte infiltration by immunohistochemistry, and leukocyte subsets by multiplex immunofluorescence. (B) Representative Movat pentachrome staining images of the cervix from control (PBS and DMSO) and preterm labor (LPS, IL-1α, and RU-486) mice with SEL. Blue staining indicates mucin, yellow indicates collagen, and red/bright red indicates muscle/fibrin. Nuclei appear as dark blue/black. (C) Representative images showing 3,3′diaminobenzidine (DAB) immunohistochemistry to detect the pan-leukocyte marker CD45 in the cervix of control (PBS and DMSO) and premature labor (LPS, IL-1α, and RU-486) mice (n = 3 per group) with SEL. (D‒F) Semi-quantification of the percentage of CD45 + cells among all cells for each study group comparison. Data are shown as dot plots where midlines indicate medians and whiskers indicate minimum and maximum values. p values for the comparisons between groups were determined by unpaired t tests. ∗p < 0.05. (G‒I) Representative merged images showing the co-localized immunofluorescence detection of neutrophils (Ly6G + cells, pink), monocytes/macrophages (F4/80 + cells, green), T cells (CD3 + cells, red), B cells (CD19 + cells, light pink), and NK cells (NCR1 + cells, yellow) in the cervices of treated mice (LPS, IL-1α, and RU-486) (n = 3 per group) with SEL. Nuclear staining is shown in blue (4′,6-diamidino-2-phenylindole; DAPI). Images were taken at 20X magnification. Scale bars represent 50μm. See also Figure S6 .

Article Snippet: Monoclonal rabbit anti-mouse CD45 antibody; clone D3F8Q , Cell Signaling Technology , Cat# 70257S, RRID: AB_2799780.

Techniques: Injection, Staining, Immunohistochemistry, Multiplex Assay, Immunofluorescence, Control, Marker, Comparison

Journal: iScience

Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth

doi: 10.1016/j.isci.2023.108341

Figure Lengend Snippet:

Article Snippet: Monoclonal rabbit anti-mouse CD45 antibody; clone D3F8Q , Cell Signaling Technology , Cat# 70257S, RRID: AB_2799780.

Techniques: Virus, Recombinant, Sequencing, Software, Staining

Journal: Cell reports

Article Title: A single-cell atlas of murine reproductive tissues during preterm labor

doi: 10.1016/j.celrep.2022.111846

Figure Lengend Snippet:

Article Snippet: Immunohistochemistry staining using the Monoclonal Rabbit Anti-Mouse CD45 (AB_2799780; clone D3F8Q, cat. no. 70257S, Cell Signaling Technology, Danvers, MA, USA) was performed using the Leica Bond Max Automatic Staining System in a peroxidase-mediated oxidation of 3,3’-diaminobenzidine (DAB) from the Bond TM Polymer Refine Detection Kit (both from Leica Microsystems, Wetzlar, Germany).

Techniques: Negative Control, Virus, Gene Expression, Software